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Alternative Names:

FRAP; FRAP1; FRAP2; RAFT1; Rapamycin target protein; kinase mTOR;

Application:

WB IHC ICC/IF IP FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:1000 IHC 1:50~1:100 ICC/IF 1:50~1:100 IP 1:30 FC 1:20

	Western blot analysis of mTOR expression in Jurkat cell lysate.
	Immunohistochemical analysis of paraffin-embedded rat liver, using mTOR Antibody.
	Overexpression of the 14-3-3γ protein in uterine leiomyoma cells results in growth retardation and increased apoptosis. -Cellular Signalling
	A brain somatic RHEB doublet mutation causes focal cortical dysplasia type II. -EXPERIMENTAL AND MOLECULAR MEDICINE
	Taurine improves low‐level inorganic arsenic‐induced insulin resistance by activating PPARγ‐mTORC2 signalling and inhibiting hepatic autophagy. -JOURNAL OF CELLULAR PHYSIOLOGY
	Prostaglandin E1 Inhibited Diabetes-Induced Phenotypic Switching of Vascular Smooth Muscle Cells Through Activating Autophagy. -Cellular Physiology and Biochemistry

Purification:

Affinity-chromatography

Specificity:

mTOR Antibody detects endogenous levels of total mTOR

Immunogen:

A synthesized peptide derived from human mTOR

Description:

An atypical kinase belonging to the PIKK family of kinases. Controls cell growth through protein synthesis regulation. Downstream of PI3K/Akt pathway and required for cell survival. Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

CY5306.pdf