Alternative Names:
MAP1LC3;LC3;LC3A;MAP1ALC3;MAP1BLC3 ;MAP1LC3A;
Application:
WB IHC ICC/IF IP FC
Reactivity:
Human Mouse Rat
Dilution:
WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50
| Western blot analysis of MAP1LC3A expression in Hela cell lysate. |
| Immunohistochemical analysis of paraffin-embedded human stomach, using MAP1LC3A Antibody. |
| Immunofluorescent analysis of Hela cells, using MAP1LC3A Antibody . |
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Purification:
Affinity-chromatography
Specificity:
MAP1LC3A Antibody detects endogenous levels of total MAP1LC3A
Immunogen:
A synthesized peptide derived from human MAP1LC3A
Description:
Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy. Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy. Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.