Alternative Names:
a actin; ACTA; ACTA1; Actin alpha skeletal muscle; Actin; actina; actine; aktin; Alpha Actin 1; Alpha skeletal muscle Actin; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; nemaline myopathy type 3;
Application:
WB IHC ICC/IF IP FC
Reactivity:
Human Mouse Rat Chicken
Dilution:
WB 1:3000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
| Western blot analysis of Actin expression in (1) Hela cell lysate; (2)Human fetal kidney lysate; (3)3T3 cell lysate; (4)Mouse spleen lysate; (5)PC-12 cell lysate; (6)Rat heart lysate. |
| Immunohistochemical analysis of paraffin-embedded mouse heart, using Actin Antibody. |
| Immunofluorescent analysis of Hela cells, using Actin Antibody. |
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Purification:
Affinity-chromatography
Specificity:
Actin Antibody detects endogenous levels of total actin protein.
Immunogen:
A synthesized peptide derived from human Actin
Description:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.