Alternative Names:
APG 5L; APG5; APG5 autophagy 5 like; APG5 like; APG5-like; Apoptosis specific protein; ASP; ATG 5; ATG5 autophagy related 5 homolog; Autophagy protein 5; hAPG5;
Application:
WB IHC ICC/IF IP
Reactivity:
Human Mouse Rat
Dilution:
WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50
| Western blot analysis of ATG5 expression in Raji cell lysate. |
| Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using ATG5 Antibody. |
| Immunofluorescent analysis of Raji cells, using ATG5 Antibody. |
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Purification:
Affinity-chromatography
Specificity:
ATG5 Antibody detects endogenous levels of ATG5
Immunogen:
A synthesized peptide derived from human ATG5
Description:
Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity.
Storage Condition and Buffer:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.