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Alternative Names:

E2AK3; EC 2.7.11.1; EIF2AK3; Eukaryotic translation initiation factor 2-alpha kinase 3 precursor; HsPEK; kinase PERK; Pancreatic eIF2-alpha kinase; PEK; PERK; PRKR-like endoplasmic reticulum kinase

Application:

WB,IHC

Reactivity:

Human,Mouse,Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200

	Western blot analysis of extracts from Jurkat cells, using PEK/PERK antibody.
	Western blot analysis of extracts from MCF-7 cells, using PEK/PERK antibody.
	Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PEK/PERK antibody.

Purification:

Affinity-chromatography

Specificity:

PERK Antibody detects endogenous levels of total PERK

Immunogen:

A synthesized peptide derived from human PERK

Description:

Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

CY2759.pdf