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Alternative Names:

FLJ36605; VIM; VIME; Vimentin

Application:

WB IHC ICC/IF FC

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:30

	Western blot analysis of Vimentin expression in HEK293 cell lysate.
	Immunohistochemical analysis of paraffin-embedded human colon, using Vimentin Antibody.
	Immunofluorescent analysis of Hela cells, using Vimentin Antibody.
	Prostaglandin E1 Inhibited Diabetes-Induced Phenotypic Switching of Vascular Smooth Muscle Cells Through Activating Autophagy. -Cellular Physiology and Biochemistry
	Overexpression of the 14-3-3γ protein in uterine leiomyoma cells results in growth retardation and increased apoptosis. -Cellular Signalling

Purification:

Affinity-chromatography

Specificity:

Vimentin Antibody detects endogenous levels of total Vimentin

Immunogen:

A synthesized peptide derived from human Vimentin

Description:

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

CY5134.pdf