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Alternative Names:

beta-4; CFEOM3A; MC1R; TBB3; TUBB3; TUBB4; Tubulin beta-3 chain; tubulin beta-4; Tubulin beta-4 chain; Tubulin beta-III; tubulin, beta 3; tubulin, beta, 4,beta tubulin;

Application:

WB

Reactivity:

Human Mouse Rat

Dilution:

WB 1:5000~1:20000

	Western blot analysis of beta Tubulin expression in (1) Jurkat cell lysate, (2) Human kidney lysate, (3) 3T3 cell lysate, (4) Mouse brain lysate, (5) C6 cell lysate, (6) Rat heart lysate.

Purification:

Affinity-chromatography

Specificity:

beta Tubulin Antibody (HRP conjugated) detects endogenous levels of total beta Tubulin

Immunogen:

A synthesized peptide derived from human beta Tubulin

Description:

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Tubulin may not be stable in certain cells. For example, expression of beta-Tubulin in adipose tissue is very low and therefore beta-Tubulin should not be used as loading control for these tissues.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

AB2002.pdf