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Alternative Names:

EC 2.7.11.1 RAC-PK-alpha Protein kinase B PKB C-AKT/EC 2.7.11.1 RAC-PK-beta Protein kinase Akt-2 Protein kinase B, beta PKB beta/EC 2.7.11.1 RAC-PK-gamma Protein kinase Akt-3 Protein kinase B, gamma PKB gamma STK-2

Application:

WB IHC

Reactivity:

Human, Mouse, Rat

Dilution:

WB 1:500-1:2000 IHC 1:50-1:200

	Western blot analysis of Akt phosphorylation expression in heatshock treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

Purification:

Affinity-chromatography

Specificity:

Phospho-Akt (Ser473) Antibody detects endogenous levels of Akt only when phosphorylated at Serine 473

Immunogen:

A synthesized peptide derived from human Akt around the phosphorylation site of Serine 473

Description:

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.