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Alternative Names:

AKT 1; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha;

Application:

WB IP

Reactivity:

Human Mouse Rat

Dilution:

WB 1:500~1:1000 IP 1:50

	Western blot analysis of Phospho-AKT1 (S124) expression in (1) MCF-7 cell treated with Alkaline Phosphatase lysate; (2) MCF-7 cell lysate.

Purification:

Affinity-chromatography

Specificity:

Phospho-AKT1 (S124) Antibody detects endogenous levels of Phospho-AKT1 (S124)

Immunogen:

A synthesized peptide derived from human AKT1

Description:

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

Storage Condition and Buffer:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

CY5926.pdf